"There cannot be economic growth without true respect for the law and a fight against criminal organisations," said Confindustria president Emma Marcegaglia.
"It's also crucial that civil society take part in this fight," she said.
Off-the-book business activities, corruption and entrenched mafia activities has heavily indebted Italy, where the economy continues to be mired in sluggish growth.
The Confindustria in June cut its 2011 and 2012 growth forecast, citing the country's "difficulty gaining momentum".
"It's important to work together to defend our country and above all to build a better future for our children," she said.